Developing Story: Blotgate
Western blots are a method of identifying whether proteins are present in an in vitro solution. They are an extremely specialised field. Blotgate is a developing story about Western blots used in an important and rather debatable paper from Pfizer.
Is the RNA intact?
Lately, scientists have been wondering if the mRNA produced by cells in response to the Pfizer and Moderna mRNA vaccines is intact, or if it may be producing partial proteins, particularly when the vaccine vials have been through a lot of weathering, temperature extremes, frozen and refrozen etc. This is called “Off-target antigen translation.”
Neurological symptoms? An Amyloidogenic protein.
Two relatively recent 2022 studies, Neurotoxic amyloidogenic peptides in the proteome of SARS-COV2: potential implications for neurological symptoms in COVID-19 Charnley et al , and Amyloidogenesis of SARS-CoV-2 Spike Protein Nyström and Hammarström found that amyloidogenic proteins present in the SARS-COV2 proteome could explain the neurological symptoms in COVID-19 patients. This may also explain neurological symptoms in some mRNA vaccine recipients - if the full protein has been broken up into parts, this partial protein could cross the blood-brain barrier and cause some kind of amyloid effect in the brain (Alzheimer’s disease is caused by misfolded amyloid proteins)
Full Spike Protein Present, say Pfizer scientists with shares in the company.
Pfizer employees recently released a study into the proteins present in the vaccine, claiming that the mRNA produced by the cells after vaccination is the full spike protein and that there are no partial RNA strands or proteins present.
Here is the paper, January 12 2023:
Characterization of BNT162b2 mRNA to Evaluate Risk of Off-Target Antigen Translation Patel et al.
This paper claims that the spike protein alone is translated by the cells, and that there are no off-target proteins present.
They have several images of Western Blots in their paper.
These blots apparently show the full spike protein is present:
What was first noticed about these is that they look too perfect: apparently doing a Western Blot in the lab is a very detailed and time-consuming process, and usually creates something more like this (an image from elsewhere in the paper that shows there are no off target mRNA expressions when the contents of the vial have been exposed to extreme heat):
Initially Jikky Leaks broke this story on Twitter:
As it turns out, these images were produced by a biotechne protein analysis tool called Jess (coincidentally since the source of this story is also called Jess- Jessica Rose) - that essentially automates the final part of the procedure, involving translating chemiluminescence into usable data.
But this begs the question, why did they use “Jess” for the full spike protein, and the traditional Western Blot procedure for showing that there are no off-target proteins present?
What makes the conclusions of the Pfizer paper debatable, of course, is the vested interests of the authors:
Dr Jessica Rose - my source for this story
In her blogs on substack and in a recent interview on the Last American Vagabond, Jessica Rose discusses blotgate, explains what it is about, and tells us that she thinks the vials that transfect the complete spike protein are probably more dangerous than the ones that contain incomplete partial protein strands.
Here is the interview:
Sources
https://jessicar.substack.com/p/a-follow-up-about-western-blots
https://jessicar.substack.com/p/characterization-of-bnt162b2-mrna
https://jessicar.substack.com/p/an-incredible-paper-was-just-published